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Tanon Science & Technology ecl western blot analysis system
Ecl Western Blot Analysis System, supplied by Tanon Science & Technology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ecl+western+blotting+analysis+system/blotting+detection+ecl+plus+system+western/pm41691874-86-6-11
Average 86 stars, based on 1 article reviews
ecl western blot analysis system - by Bioz Stars, 2026-09
86/100 stars

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Related Articles

Western Blot:

Article Title: DCAF13 is essential for mouse uterine function and fertility
Article Snippet: Following this, the membrane was washed three times with TBST and a horseradish peroxidase enzyme-linked secondary antibody (1:5000 dilution in 1% BSA, HuaBio) was added and incubated at room temperature for 1 h. The membrane was subsequently rinsed three times with TBST. .. The protein bands were visualized using an enhanced chemiluminescence (ECL) Western Blotting Kit (Millipore, Bedford, MA, USA) and an ECL Western Blotting analysis system (Tanon 5500 Multi, Shanghai, China). ..

Article Title: A multiepitope vaccine encoding four Eimeria epitopes with PLGA nanospheres: a novel vaccine candidate against coccidiosis in laying chickens
Article Snippet: Briefly, the purified NSLC protein was first analysed in a 12% SDS–PAGE gel and then transferred to a polyvinylidene difluoride (PVDF) membrane (Immobilon-PSQ, Millipore, Billerica, MA, USA). .. Then, the blocking buffer, namely, TBST (TBS containing 0.5% Tween 20) containing 5% ( w / v ) skimmed milk powder, was used to block nonspecific binding sites at 37 °C for 2 h. Next, the membrane was rinsed in TBST for 5 min and incubated in chicken sera (1:100 dilution) at 37 °C for 2 h. After being washed three times in TBST, the membrane was then incubated with HRP-conjugated goat anti-rat IgG (1:5000 dilution, eBioscience, San Diego, USA) in TBST at 37 °C for 1 h. Finally, the membrane was visualized by the chemiluminescence ECL Western blotting analysis system (Tanon, Shanghai, China). .. To synthesize the NSLC-FA emulsions (water-in-oil emulsion), complete Freund’s adjuvant (CFA, Sigma, Saint Louis, MO, USA) was added to an equal volume of NSLC protein solution.

Article Title: AMPK/Drp1 pathway mediates Streptococcus uberis-Induced mitochondrial dysfunction.
Article Snippet: Excessive production of reactive oxygen species (ROS) leads to oxidative stress in host cells and affects the progress of disease.. Mitochondria are an important source of ROS and their dysfunction is closely related to ROS production.. S. uberis is a common causative agent of mastitis.

Article Title: DCAF13 is essential for mouse uterine function and fertility.
Article Snippet: Following this, the membrane was washed three times with TBST and a horseradish peroxidase enzyme-linked secondary antibody (1:5000 dilution in 1% BSA, HuaBio) was added and incubated at room temperature for 1 h. The membrane was subsequently rinsed three times with TBST. .. The protein bands were visualized using an enhanced chemiluminescence (ECL) Western Blotting Kit (Millipore, Bedford, MA, USA) and an ECL Western Blotting analysis system (Tanon Fig. 2 The thin endometrium and insufficient proliferation of endometrial cells in adult Dcaf13 cKO female mice. ..

Blocking Assay:

Article Title: A multiepitope vaccine encoding four Eimeria epitopes with PLGA nanospheres: a novel vaccine candidate against coccidiosis in laying chickens
Article Snippet: Briefly, the purified NSLC protein was first analysed in a 12% SDS–PAGE gel and then transferred to a polyvinylidene difluoride (PVDF) membrane (Immobilon-PSQ, Millipore, Billerica, MA, USA). .. Then, the blocking buffer, namely, TBST (TBS containing 0.5% Tween 20) containing 5% ( w / v ) skimmed milk powder, was used to block nonspecific binding sites at 37 °C for 2 h. Next, the membrane was rinsed in TBST for 5 min and incubated in chicken sera (1:100 dilution) at 37 °C for 2 h. After being washed three times in TBST, the membrane was then incubated with HRP-conjugated goat anti-rat IgG (1:5000 dilution, eBioscience, San Diego, USA) in TBST at 37 °C for 1 h. Finally, the membrane was visualized by the chemiluminescence ECL Western blotting analysis system (Tanon, Shanghai, China). .. To synthesize the NSLC-FA emulsions (water-in-oil emulsion), complete Freund’s adjuvant (CFA, Sigma, Saint Louis, MO, USA) was added to an equal volume of NSLC protein solution.

Binding Assay:

Article Title: A multiepitope vaccine encoding four Eimeria epitopes with PLGA nanospheres: a novel vaccine candidate against coccidiosis in laying chickens
Article Snippet: Briefly, the purified NSLC protein was first analysed in a 12% SDS–PAGE gel and then transferred to a polyvinylidene difluoride (PVDF) membrane (Immobilon-PSQ, Millipore, Billerica, MA, USA). .. Then, the blocking buffer, namely, TBST (TBS containing 0.5% Tween 20) containing 5% ( w / v ) skimmed milk powder, was used to block nonspecific binding sites at 37 °C for 2 h. Next, the membrane was rinsed in TBST for 5 min and incubated in chicken sera (1:100 dilution) at 37 °C for 2 h. After being washed three times in TBST, the membrane was then incubated with HRP-conjugated goat anti-rat IgG (1:5000 dilution, eBioscience, San Diego, USA) in TBST at 37 °C for 1 h. Finally, the membrane was visualized by the chemiluminescence ECL Western blotting analysis system (Tanon, Shanghai, China). .. To synthesize the NSLC-FA emulsions (water-in-oil emulsion), complete Freund’s adjuvant (CFA, Sigma, Saint Louis, MO, USA) was added to an equal volume of NSLC protein solution.

Membrane:

Article Title: A multiepitope vaccine encoding four Eimeria epitopes with PLGA nanospheres: a novel vaccine candidate against coccidiosis in laying chickens
Article Snippet: Briefly, the purified NSLC protein was first analysed in a 12% SDS–PAGE gel and then transferred to a polyvinylidene difluoride (PVDF) membrane (Immobilon-PSQ, Millipore, Billerica, MA, USA). .. Then, the blocking buffer, namely, TBST (TBS containing 0.5% Tween 20) containing 5% ( w / v ) skimmed milk powder, was used to block nonspecific binding sites at 37 °C for 2 h. Next, the membrane was rinsed in TBST for 5 min and incubated in chicken sera (1:100 dilution) at 37 °C for 2 h. After being washed three times in TBST, the membrane was then incubated with HRP-conjugated goat anti-rat IgG (1:5000 dilution, eBioscience, San Diego, USA) in TBST at 37 °C for 1 h. Finally, the membrane was visualized by the chemiluminescence ECL Western blotting analysis system (Tanon, Shanghai, China). .. To synthesize the NSLC-FA emulsions (water-in-oil emulsion), complete Freund’s adjuvant (CFA, Sigma, Saint Louis, MO, USA) was added to an equal volume of NSLC protein solution.

Incubation:

Article Title: A multiepitope vaccine encoding four Eimeria epitopes with PLGA nanospheres: a novel vaccine candidate against coccidiosis in laying chickens
Article Snippet: Briefly, the purified NSLC protein was first analysed in a 12% SDS–PAGE gel and then transferred to a polyvinylidene difluoride (PVDF) membrane (Immobilon-PSQ, Millipore, Billerica, MA, USA). .. Then, the blocking buffer, namely, TBST (TBS containing 0.5% Tween 20) containing 5% ( w / v ) skimmed milk powder, was used to block nonspecific binding sites at 37 °C for 2 h. Next, the membrane was rinsed in TBST for 5 min and incubated in chicken sera (1:100 dilution) at 37 °C for 2 h. After being washed three times in TBST, the membrane was then incubated with HRP-conjugated goat anti-rat IgG (1:5000 dilution, eBioscience, San Diego, USA) in TBST at 37 °C for 1 h. Finally, the membrane was visualized by the chemiluminescence ECL Western blotting analysis system (Tanon, Shanghai, China). .. To synthesize the NSLC-FA emulsions (water-in-oil emulsion), complete Freund’s adjuvant (CFA, Sigma, Saint Louis, MO, USA) was added to an equal volume of NSLC protein solution.

Article Title: AMPK/Drp1 pathway mediates Streptococcus uberis-Induced mitochondrial dysfunction.
Article Snippet: Excessive production of reactive oxygen species (ROS) leads to oxidative stress in host cells and affects the progress of disease.. Mitochondria are an important source of ROS and their dysfunction is closely related to ROS production.. S. uberis is a common causative agent of mastitis.



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